Artemia salina Linn. (Brine Shrimp) Lethality Assay of Tinospora rumphii Boerl (Panyawan) Leaves and Stems Crude Ethanolic Extract

Tinospora rumphii Boerl commonly known as Panyawan plant is a rising, dioecious vine reaching a height of 4 to 10 meters. Stems are up to 1 centimeter bushy and somewhat thick, with spread swellings. Leaves are thin, oval, 6 to 12 centimeters long, and 7 to 12 centimeters widespread, with pointy and shorten or somewhat core-formed grounded, smooth and shining and the name itself suggests the primary purpose of such plant pro-life or to give life. The present study investigated the lethal concentration of the leaves and stem of Panyawan. Artemia salina Linn. were equally divided into 4 different concentrations namely 20mg/ml, 40mg/ml, 60mg/ml, 100mg/ml against the negative (-) and the positive (+) control. The lethal test was determined after 3 hours. The result shows that there is no significant difference among the positive group and the leaf and stem concentration of 100% and shows that both the leaf and stem having a concentration of 100% has comparable lethal effects to the positive Potassium dichromate. Both the stems and the leaf crude extract of the Tinospora rumphii Boerl have, therefore, a significant lethal properties on the Artemia salina tests subjects. As the concentration of Tinospora rumphii Boerl increases, the lethality effect of the plant extracts on Artemia salina Linn also increases.

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Keywords: Artemia salina Linn, Tinospora rumphii Boerl, water, potassium dichromate, lethality assay.