Larvicidal Bioassay of Chromolaena Odorata Linn. (Hagonoy) Leaves Solution Against Mosquito Larvae

Chromolaena odorata Linn. commonly known as Hagonoy; aside from its wound healing property, Hagonoy finds its application as a larvicide against mosquito larvae. Early studies showed its larvicidal activity. The present study investigated the larvicidal activity of the plant leaves extracts formulated into solution using acetone against larvae of mosquito. Three groups namely; negative control, positive control, and experimental group, are obtained by dividing a total of 225 mosquito larvae. Each group contains three replicates containing 15 larvae. The groups were treated as follows: 5ml of 1% acetone stock solution for negative control, dissolved OL pellets for positive control, and sufficient amount of 1% stock solution of the Hagonoy leaves the solution to obtain the desired concentrations for the experimental group. The number of larvae death was recorded every 7 am, 1 pm, and 6 pm for two days. The gathered data were analyzed statistically. The findings showed that the solution of Hagonoy leaves extract has no larvicidal property and its effect is significantly different with the positive control. Thus, it is not a potential larvicide.

Keywords: Chromolaena odorata Linn., Aedes aegypti, larvicide