Evaluation of Anti-Inflammatory Activity of Tinospora cordifolia Willd. (Panyawan) Leaf Extracts by In-Vitro Method

The objective of present work is to evaluate the in-vitro anti-inflammatory activity of Tinospora cordifolia Willd. (Panyawan) against denaturation of proteins. Denaturation of tissue proteins is one of the well-documented causes of inflammation. The ethanolic leaf extract of the plant was studied for in-vitro anti-inflammatory activity by egg albumin denaturation method. Different concentration of T. cordifolia was incubated with egg albumin in specified experimental conditions and subjected to determination of absorbance to retrieve the in-vitro anti-inflammatory property using aspirin as reference standard against denaturation of protein. The present findings resulted that at the ethanolic leaf extract concentration of 100mg/mL, 200mg/mL and 500mg/mL showed 82.857, 87.879, and 92.5% inhibition of egg albumin denaturation, whereas, aspirin in 100mg/mL, 200g/mL and 500mg/mL concentration showed 81.25, 85.366, and 87.541% inhibition of egg albumin denaturation. It was found that ethanolic extract of T. cordifolia Willd. exhibited maximum percentage of inhibition and therefore, more potent in inhibition of egg albumin denaturation method than aspirin. Finally, from results it can be concluded that Tinospora cordifolia Willd. (Panyawan) posessed marked in-vitro anti-inflammatory activity. The further extensive research resulted T. cordifolia Willd. (Panyawan) may be a potential alternative anti-inflammatory agent.

Keywords: Aspirin, anti-inflammatory, in-vitro methods, protein denaturation, absorbance, egg albumin